flower: Analyze 454 flowgrams (.SFF files)

flower - FLOWgram ExtractoR tools

This is now obsolete, please install the biosff library (which includes the flower exectuable) instead.

The flower executable reads files in SFF-format and produces various output, including sequences with quality, or flowgram data in tabular format. By default, it outputs a textual representation of the data in the SFF-file, much like sffinfo from Roche does.

The flowselect executable extracts reads from SFF-files, generating a new SFF-file with a subset of the reads based on various quality criteria.

The flowt program removes (artificial) duplicates from SFF files. It's currently a work in progress, but included if you'd like to play with it. It's faster than other approaches (e.g. CD-HIT), and ought to be more sensitive and specific, but this needs to be proven.

Downloads

• flower-0.7.2.tar.gz [browse] (Cabal source package)
• Package description (as included in the package)

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• KetilMalde

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