module Bio.Bam.Rec (
BamRaw,
bamRaw,
virt_offset,
raw_data,
BamRec(..),
unpackBam,
nullBamRec,
getMd,
Cigar(..),
CigOp(..),
alignedLength,
Nucleotides(..), Vector_Nucs_half,
Extensions, Ext(..),
extAsInt, extAsString, setQualFlag,
deleteE, insertE, updateE, adjustE,
isPaired,
isProperlyPaired,
isUnmapped,
isMateUnmapped,
isReversed,
isMateReversed,
isFirstMate,
isSecondMate,
isAuxillary,
isFailsQC,
isDuplicate,
isTrimmed,
isMerged,
type_mask,
progressBam,
Word32
) where
import Bio.Base
import Bio.Bam.Header
import Bio.Iteratee
import Control.Monad
import Control.Monad.Primitive ( unsafePrimToPrim, unsafeInlineIO )
import Control.Applicative
import Data.Bits ( Bits, testBit, shiftL, shiftR, (.&.), (.|.) )
import Data.ByteString ( ByteString )
import Data.Int ( Int32, Int16, Int8 )
import Data.Ix
import Data.String ( fromString )
import Data.Word ( Word32, Word16 )
import Foreign.ForeignPtr
import Foreign.Marshal.Alloc ( alloca )
import Foreign.Storable ( peek, poke, peekByteOff, pokeByteOff, Storable(..) )
import System.IO.Unsafe ( unsafeDupablePerformIO )
import qualified Data.ByteString as B
import qualified Data.ByteString.Char8 as S
import qualified Data.ByteString.Internal as B
import qualified Data.ByteString.Unsafe as B
import qualified Data.Vector.Generic as V
import qualified Data.Vector.Generic.Mutable as VM
import qualified Data.Vector.Storable as VS
import qualified Data.Vector.Unboxed as U
data Cigar = !CigOp :* !Int deriving (Eq, Ord)
infix 9 :*
data CigOp = Mat | Ins | Del | Nop | SMa | HMa | Pad
deriving ( Eq, Ord, Enum, Show, Bounded, Ix )
instance Show Cigar where
showsPrec _ (op :* num) = shows num . (:) (S.index "MIDNSHP" (fromEnum op))
instance Storable Cigar where
sizeOf _ = 4
alignment _ = 1
peek p = do w0 <- peekByteOff p 0 :: IO Word8
w1 <- peekByteOff p 1 :: IO Word8
w2 <- peekByteOff p 2 :: IO Word8
w3 <- peekByteOff p 3 :: IO Word8
let w = fromIntegral w0 `shiftL` 0 .|. fromIntegral w1 `shiftL` 8 .|.
fromIntegral w2 `shiftL` 16 .|. fromIntegral w3 `shiftL` 24
return $ toEnum (w .&. 0xf) :* shiftR w 4
poke p (op :* num) = do pokeByteOff p 0 (fromIntegral $ shiftR w 0 :: Word8)
pokeByteOff p 1 (fromIntegral $ shiftR w 8 :: Word8)
pokeByteOff p 2 (fromIntegral $ shiftR w 16 :: Word8)
pokeByteOff p 3 (fromIntegral $ shiftR w 24 :: Word8)
where
w = fromEnum op .|. shiftL num 4
alignedLength :: V.Vector v Cigar => v Cigar -> Int
alignedLength = V.foldl' (\a -> (a +) . l) 0
where l (op :* n) = if op == Mat || op == Del || op == Nop then n else 0
data BamRec = BamRec {
b_qname :: Seqid,
b_flag :: Int,
b_rname :: Refseq,
b_pos :: Int,
b_mapq :: Qual,
b_cigar :: VS.Vector Cigar,
b_mrnm :: Refseq,
b_mpos :: Int,
b_isize :: Int,
b_seq :: Vector_Nucs_half Nucleotides,
b_qual :: VS.Vector Qual,
b_exts :: Extensions,
b_virtual_offset :: FileOffset
} deriving Show
nullBamRec :: BamRec
nullBamRec = BamRec {
b_qname = S.empty,
b_flag = flagUnmapped,
b_rname = invalidRefseq,
b_pos = invalidPos,
b_mapq = Q 0,
b_cigar = VS.empty,
b_mrnm = invalidRefseq,
b_mpos = invalidPos,
b_isize = 0,
b_seq = V.empty,
b_qual = VS.empty,
b_exts = [],
b_virtual_offset = 0
}
getMd :: BamRec -> Maybe [MdOp]
getMd r = case lookup "MD" $ b_exts r of
Just (Text mdfield) -> readMd mdfield
Just (Char mdfield) -> readMd $ B.singleton mdfield
_ -> Nothing
data Vector_Nucs_half a = Vector_Nucs_half !Int !Int !(ForeignPtr Word8)
data MVector_Nucs_half s a = MVector_Nucs_half !Int !Int !(ForeignPtr Word8)
type instance V.Mutable Vector_Nucs_half = MVector_Nucs_half
instance V.Vector Vector_Nucs_half Nucleotides where
basicUnsafeFreeze (MVector_Nucs_half o l fp) = return $ Vector_Nucs_half o l fp
basicUnsafeThaw (Vector_Nucs_half o l fp) = return $ MVector_Nucs_half o l fp
basicLength (Vector_Nucs_half _ l _) = l
basicUnsafeSlice s l (Vector_Nucs_half o _ fp) = Vector_Nucs_half (o + s) l fp
basicUnsafeIndexM (Vector_Nucs_half o _ fp) i
| even (o+i) = return . Ns $ (b `shiftR` 4) .&. 0xF
| otherwise = return . Ns $ b .&. 0xF
where !b = unsafeInlineIO $ withForeignPtr fp $ \p -> peekByteOff p ((o+i) `shiftR` 1)
instance VM.MVector MVector_Nucs_half Nucleotides where
basicLength (MVector_Nucs_half _ l _) = l
basicUnsafeSlice s l (MVector_Nucs_half o _ fp) = MVector_Nucs_half (o + s) l fp
basicOverlaps (MVector_Nucs_half _ _ fp1) (MVector_Nucs_half _ _ fp2) = fp1 == fp2
basicUnsafeNew l = unsafePrimToPrim $ MVector_Nucs_half 0 l <$> mallocForeignPtrBytes ((l+1) `shiftR` 1)
basicUnsafeRead (MVector_Nucs_half o _ fp) i
| even (o+i) = liftM (Ns . (.&.) 0xF . (`shiftR` 4)) b
| otherwise = liftM (Ns . (.&.) 0xF ) b
where b = unsafePrimToPrim $ withForeignPtr fp $ \p -> peekByteOff p ((o+i) `shiftR` 1)
basicUnsafeWrite (MVector_Nucs_half o _ fp) i (Ns x) =
unsafePrimToPrim $ withForeignPtr fp $ \p -> do
y <- peekByteOff p ((o+i) `shiftR` 1)
let y' | even (o+i) = x `shiftL` 4 .|. y .&. 0x0F
| otherwise = x .|. y .&. 0xF0
pokeByteOff p ((o+i) `shiftR` 1) y'
instance Show (Vector_Nucs_half Nucleotides) where
show = show . V.toList
data BamRaw = BamRaw { virt_offset :: !FileOffset
, raw_data :: !S.ByteString }
bamRaw :: FileOffset -> S.ByteString -> BamRaw
bamRaw o s = if good then BamRaw o s else error $ "broken BAM record " ++ show (S.length s, m) ++ show m
where
good | S.length s < 32 = False
| otherwise = S.length s >= sum m
m = [ 32, l_rnm, l_seq, (l_seq+1) `div` 2, l_cig * 4 ]
l_rnm = fromIntegral (B.unsafeIndex s 8) 1
l_cig = fromIntegral (B.unsafeIndex s 12) .|. fromIntegral (B.unsafeIndex s 13) `shiftL` 8
l_seq = fromIntegral (B.unsafeIndex s 16) .|. fromIntegral (B.unsafeIndex s 17) `shiftL` 8 .|.
fromIntegral (B.unsafeIndex s 18) `shiftL` 16 .|. fromIntegral (B.unsafeIndex s 19) `shiftL` 24
unpackBam :: BamRaw -> BamRec
unpackBam br = BamRec {
b_rname = Refseq $ getInt32 0,
b_pos = getInt32 4,
b_mapq = Q $ getInt8 9,
b_flag = getInt16 14,
b_mrnm = Refseq $ getInt32 20,
b_mpos = getInt32 24,
b_isize = fromIntegral (getInt32 28 :: Int32),
b_qname = B.unsafeTake l_read_name $ B.unsafeDrop 32 $ raw_data br,
b_cigar = VS.unsafeCast $ VS.unsafeFromForeignPtr fp (off0+off_c) (4*l_cigar),
b_seq = Vector_Nucs_half (2 * (off_s+off0)) l_seq fp,
b_qual = VS.unsafeCast $ VS.unsafeFromForeignPtr fp (off0+off_q) l_seq,
b_exts = unpackExtensions $ S.drop off_e $ raw_data br,
b_virtual_offset = virt_offset br }
where
(fp, off0, _) = B.toForeignPtr $ raw_data br
off_c = 33 + l_read_name
off_s = off_c + 4 * l_cigar
off_q = off_s + (l_seq + 1) `div` 2
off_e = off_q + l_seq
l_read_name = getInt8 8 1
l_seq = getInt32 16
l_cigar = getInt16 12
getInt8 :: (Num a, Bits a) => Int -> a
getInt8 o = fromIntegral (B.unsafeIndex (raw_data br) o)
getInt16 :: (Num a, Bits a) => Int -> a
getInt16 o = fromIntegral (B.unsafeIndex (raw_data br) o) .|.
fromIntegral (B.unsafeIndex (raw_data br) $ o+1) `shiftL` 8
getInt32 :: (Num a, Bits a) => Int -> a
getInt32 o = fromIntegral (B.unsafeIndex (raw_data br) $ o+0) .|.
fromIntegral (B.unsafeIndex (raw_data br) $ o+1) `shiftL` 8 .|.
fromIntegral (B.unsafeIndex (raw_data br) $ o+2) `shiftL` 16 .|.
fromIntegral (B.unsafeIndex (raw_data br) $ o+3) `shiftL` 24
type Extensions = [( BamKey, Ext )]
deleteE :: BamKey -> Extensions -> Extensions
deleteE k = filter ((/=) k . fst)
insertE :: BamKey -> Ext -> Extensions -> Extensions
insertE k v = (:) (k,v)
updateE :: BamKey -> Ext -> Extensions -> Extensions
updateE k v = insertE k v . deleteE k
adjustE :: (Ext -> Ext) -> BamKey -> Extensions -> Extensions
adjustE _ _ [ ] = []
adjustE f k ((k',v):es) | k == k' = (k', f v) : es
| otherwise = (k', v) : adjustE f k es
data Ext = Int Int | Float Float | Text ByteString | Bin ByteString | Char Word8
| IntArr (U.Vector Int) | FloatArr (U.Vector Float)
deriving (Show, Eq, Ord)
unpackExtensions :: ByteString -> Extensions
unpackExtensions = go
where
go s | S.length s < 4 = []
| otherwise = let key = fromString [ S.index s 0, S.index s 1 ]
in case S.index s 2 of
'Z' -> case S.break (== '\0') (S.drop 3 s) of (l,r) -> (key, Text l) : go (S.drop 1 r)
'H' -> case S.break (== '\0') (S.drop 3 s) of (l,r) -> (key, Bin l) : go (S.drop 1 r)
'A' -> (key, Char (B.index s 3)) : go (S.drop 4 s)
'B' -> let tp = S.index s 3
n = getInt 'I' (S.drop 4 s)
in case tp of
'f' -> (key, FloatArr (U.fromListN (n+1) [ getFloat (S.drop i s) | i <- [8, 12 ..] ]))
: go (S.drop (12+4*n) s)
_ -> (key, IntArr (U.fromListN (n+1) [ getInt tp (S.drop i s) | i <- [8, 8 + size tp ..] ]))
: go (S.drop (8 + size tp * (n+1)) s)
'f' -> (key, Float (getFloat (S.drop 3 s))) : go (S.drop 7 s)
tp -> (key, Int (getInt tp (S.drop 3 s))) : go (S.drop (3 + size tp) s)
size 'C' = 1
size 'c' = 1
size 'S' = 2
size 's' = 2
size 'I' = 4
size 'i' = 4
size 'f' = 4
size _ = 0
getInt 'C' s | S.length s >= 1 = fromIntegral (fromIntegral (B.index s 0) :: Word8)
getInt 'c' s | S.length s >= 1 = fromIntegral (fromIntegral (B.index s 0) :: Int8)
getInt 'S' s | S.length s >= 2 = fromIntegral (i :: Word16)
where i = fromIntegral (B.index s 0) .|. fromIntegral (B.index s 1) `shiftL` 8
getInt 's' s | S.length s >= 2 = fromIntegral (i :: Int16)
where i = fromIntegral (B.index s 0) .|. fromIntegral (B.index s 1) `shiftL` 8
getInt 'I' s | S.length s >= 4 = fromIntegral (i :: Word32)
where i = fromIntegral (B.index s 0) .|. fromIntegral (B.index s 1) `shiftL` 8 .|.
fromIntegral (B.index s 2) `shiftL` 16 .|. fromIntegral (B.index s 3) `shiftL` 24
getInt 'i' s | S.length s >= 4 = fromIntegral (i :: Int32)
where i = fromIntegral (B.index s 0) .|. fromIntegral (B.index s 1) `shiftL` 8 .|.
fromIntegral (B.index s 2) `shiftL` 16 .|. fromIntegral (B.index s 3) `shiftL` 24
getInt _ _ = 0
getFloat s = unsafeDupablePerformIO $ alloca $ \buf ->
pokeByteOff buf 0 (getInt 'I' s :: Word32) >> peek buf
isPaired, isProperlyPaired, isUnmapped, isMateUnmapped, isReversed,
isMateReversed, isFirstMate, isSecondMate, isAuxillary, isFailsQC,
isDuplicate, isTrimmed, isMerged :: BamRec -> Bool
isPaired = flip testBit 0 . b_flag
isProperlyPaired = flip testBit 1 . b_flag
isUnmapped = flip testBit 2 . b_flag
isMateUnmapped = flip testBit 3 . b_flag
isReversed = flip testBit 4 . b_flag
isMateReversed = flip testBit 5 . b_flag
isFirstMate = flip testBit 6 . b_flag
isSecondMate = flip testBit 7 . b_flag
isAuxillary = flip testBit 8 . b_flag
isFailsQC = flip testBit 9 . b_flag
isDuplicate = flip testBit 10 . b_flag
isTrimmed = flip testBit 0 . extAsInt 0 "FF"
isMerged = flip testBit 1 . extAsInt 0 "FF"
type_mask :: Int
type_mask = flagFirstMate .|. flagSecondMate .|. flagPaired
extAsInt :: Int -> BamKey -> BamRec -> Int
extAsInt d nm br = case lookup nm (b_exts br) of Just (Int i) -> i ; _ -> d
extAsString :: BamKey -> BamRec -> ByteString
extAsString nm br = case lookup nm (b_exts br) of
Just (Char c) -> B.singleton c
Just (Text s) -> s
_ -> B.empty
setQualFlag :: Char -> BamRec -> BamRec
setQualFlag c br = br { b_exts = updateE "ZQ" (Text s') $ b_exts br }
where
s = extAsString "ZQ" br
s' = if c `S.elem` s then s else c `S.cons` s
progressBam :: MonadIO m => String -> (String -> IO ()) -> Refs -> Enumeratee [BamRaw] [BamRaw] m a
progressBam = progressPos (\br -> case unpackBam br of b -> (b_rname b, b_pos b))