module Bio.GenbankParser (
parseGenbank,
readGenbank,
module Bio.GenbankData
) where
import Bio.GenbankData
import Text.ParserCombinators.Parsec
import Text.ParserCombinators.Parsec.Token
import Text.ParserCombinators.Parsec.Language (emptyDef)
import Control.Monad
import Data.List
import Data.List.Split (splitOn)
import Data.Maybe
import Bio.Core.Sequence
import qualified Data.ByteString.Lazy.Char8 as L
genParserGenbank :: GenParser Char st Genbank
genParserGenbank = do
string "LOCUS"
many1 space
locus <- many1 (noneOf " ")
many1 space
length <- many1 (noneOf " ")
string " bp"
many1 space
moleculeType <- many1 (noneOf " ")
many1 space
circular <- many1 (noneOf " ")
many1 space
division <- many1 (noneOf " ")
many1 space
creationDate <- many1 (noneOf "\n")
newline
definition <- genParserField "DEFINITION" "ACCESSION"
accession <- genParserField "ACCESSION" "VERSION"
string "VERSION"
many1 space
version <- many1 (noneOf " ")
many1 space
geneIdentifier <- many1 (noneOf "\n")
newline
dblink <- genParserField "DBLINK" "KEYWORDS"
keywords <- genParserField "KEYWORDS" "SOURCE"
source <- genParserField "SOURCE" "ORGANISM"
organism <- genParserField "ORGANISM" "REFERENCE"
references <- many1 genParserReference
comment <- genParserField "COMMENT" "FEATURES"
string "FEATURES"
many1 space
string "Location/Qualifiers"
newline
features <- many genParserFeature
contig <- optionMaybe (try (genParserField "CONTIG" "ORIGIN"))
string "ORIGIN"
many (string " ")
newline
origin <- many1 genParserOriginSequence
string "//"
newline
return $ Genbank (L.pack locus) (readInt length) (L.pack moleculeType) (L.pack circular) (L.pack division) (L.pack creationDate) (L.pack definition) (L.pack accession) (L.pack version) (L.pack geneIdentifier) (L.pack dblink) (L.pack keywords) (L.pack source) (L.pack organism) references (L.pack comment) features contig (origintoSeqData origin)
genParserFeature :: GenParser Char st Feature
genParserFeature = do
string " "
featureType <- choice [try (string "gene") , try (string "repeat_region"), try (string "source")]
many1 space
genericFeatureCoordinates <- choice [genParserCoordinatesSet "join", genParserCoordinatesSet "order"]
attibutes <- many (try genParserAttributes)
subFeatures <- many (try genParserSubFeature)
choice [try geneAhead, try repeatAhead, try (lookAhead (string "CONTIG")), try (lookAhead (string "ORIGIN"))]
return $ Feature (L.pack featureType) genericFeatureCoordinates attibutes subFeatures
genParserAttributes :: GenParser Char st Attribute
genParserAttributes = choice [try genParserAttribute, try genParseGOattribute, try genParserFlagAttribute]
genParserAttribute :: GenParser Char st Attribute
genParserAttribute = do
many1 space
string "/"
notFollowedBy (string "translation")
fieldName <- many1 (noneOf "=")
string "=\""
stringField <- many1 (noneOf "\"")
string "\""
newline
return $ Field (L.pack fieldName) (L.pack stringField)
genParserSubFeature :: GenParser Char st SubFeature
genParserSubFeature = do
string " "
notFollowedBy (choice [string "gene", string "repeat_region", string "source"])
subFeatureType <- many1 (noneOf " ")
many1 space
subFeatureCoordinates <- choice [genParserCoordinatesSet "join", genParserCoordinatesSet "order"]
attibutes <- many (try genParserAttributes)
subFeatureTranslation <- optionMaybe (try (parseStringField "translation"))
return $ SubFeature (L.pack subFeatureType) subFeatureCoordinates attibutes (translationtoSeqData subFeatureTranslation)
genParseGOattribute :: GenParser Char st Attribute
genParseGOattribute = do
many1 space
string "/GO_"
goType <- many1 (noneOf "=")
string "=\""
goId <- many1 (noneOf "-")
string "-"
goName <- many1 (noneOf "\"")
string "\""
newline
return $ GOattribute (L.pack goType) (L.pack goId) (L.pack goName)
genParserFlagAttribute :: GenParser Char st Attribute
genParserFlagAttribute = do
many1 space
string "/"
notFollowedBy (string "translation")
flagType <- many1 (noneOf "\n")
newline
return $ Flag (L.pack flagType)
parseGenbank :: String -> Either ParseError Genbank
parseGenbank = parse genParserGenbank "genParserGenbank"
readGenbank :: String -> IO (Either ParseError Genbank)
readGenbank = parseFromFile genParserGenbank
genParserField :: String -> String -> GenParser Char st String
genParserField fieldStart fieldEnd = do
string fieldStart
many1 space
manyTill anyChar (try (lookAhead (string fieldEnd)))
genParserOriginSequence :: GenParser Char st String
genParserOriginSequence = do
many1 space
many1 (noneOf " ")
space
originSequence <- many1 (noneOf "\n")
newline
return originSequence
genParserOriginSlice :: GenParser Char st OriginSlice
genParserOriginSlice = do
many1 space
originIndex <- many1 (noneOf " ")
space
originSequence <- many1 (noneOf "\n")
newline
return $ OriginSlice (readInt originIndex) originSequence
genParserReference :: GenParser Char st Reference
genParserReference = do
string "REFERENCE"
many1 space
index <- many1 digit
many (string " ")
optional (try (string "(bases"))
many (string " ")
baseFrom <- optionMaybe (try (many1 digit))
many (string " ")
optional (try (string "to"))
many (string " ")
baseTo <- optionMaybe (try (many1 digit))
optional (try (string ")"))
newline
many1 space
authors <- choice [genParserField "AUTHORS" "TITLE", genParserField "CONSRTM" "TITLE"]
title <- genParserField "TITLE" "JOURNAL"
journal <- choice [try (genParserField "JOURNAL" "REFERENCE"), genParserField "JOURNAL" "COMMENT"]
return $ Reference (readInt index) (liftM readInt baseFrom) (liftM readInt baseTo) authors title journal Nothing Nothing
parseFlag :: String -> GenParser Char st Char
parseFlag flagString = do
many1 space
flag <- string ('/' : flagString)
newline
geneAhead = lookAhead (string " gene")
repeatAhead = lookAhead (string " repeat")
origintoSeqData :: [String] -> SeqData
origintoSeqData originInput = SeqData (L.pack (filter (\nuc -> nuc /= '\n' && (nuc /= ' ')) (concat originInput)))
translationtoSeqData :: Maybe String -> Maybe SeqData
translationtoSeqData translationInput
| isJust translationInput = Just (SeqData (L.pack (filter (\aminoacid -> (aminoacid /= '\n') && (aminoacid /= ' ') ) (fromJust translationInput))))
| otherwise = Nothing
genParserCoordinates :: GenParser Char st Coordinates
genParserCoordinates = do
coordinates <- choice [try genParserForwardCoordinates, try genParserComplementCoordinates]
return coordinates
genParserCoordinatesSet :: String -> GenParser Char st CoordinateSet
genParserCoordinatesSet prefix = do
coordinates <- choice [try (many1 genParserForwardCoordinates), try (many1 genParserComplementCoordinates), try (genParserForwardPrefix prefix), try (genParserComplementPrefix prefix)]
return $ CoordinateSet coordinates (Just prefix)
genParserForwardPrefix :: String -> GenParser Char st [Coordinates]
genParserForwardPrefix prefix = do
string (prefix ++ "(")
coordinates <- many1 genParserForwardPrefixCoordinates
string ")"
return coordinates
genParserForwardPrefixCoordinates :: GenParser Char st Coordinates
genParserForwardPrefixCoordinates = do
coordinateFromEqualitySymbol <- optionMaybe (try (oneOf "><"))
coordinateFrom <- many1 digit
optional (oneOf "><")
string "."
string "."
coordinateToEqualitySymbol <- optionMaybe (try (oneOf "><"))
coordinateTo <- many1 digit
optional (choice [try (string ",\n"),try (string ",")])
optional (many1 (string " "))
return $ Coordinates (readInt coordinateFrom) coordinateFromEqualitySymbol (readInt coordinateTo) coordinateToEqualitySymbol True
genParserComplementPrefix :: String -> GenParser Char st [Coordinates]
genParserComplementPrefix prefix = do
string "complement("
string (prefix ++ "(")
coordinates <- many1 genParserForwardPrefixCoordinates
string ")"
string ")"
newline
return (setComplement False coordinates)
genParserForwardCoordinates :: GenParser Char st Coordinates
genParserForwardCoordinates = do
coordinateFromEqualitySymbol <- optionMaybe (try (oneOf "><"))
coordinateFrom <- many1 digit
optional (oneOf "><")
string "."
string "."
coordinateToEqualitySymbol <- optionMaybe (try (oneOf "><"))
coordinateTo <- many1 digit
newline
return $ Coordinates (readInt coordinateFrom) coordinateFromEqualitySymbol (readInt coordinateTo) coordinateToEqualitySymbol False
genParserComplementCoordinates :: GenParser Char st Coordinates
genParserComplementCoordinates = do
string "complement("
coordinateFromEqualitySymbol <- optionMaybe (try (oneOf "><"))
coordinateFrom <- many1 digit
optional (oneOf "><")
string "."
string "."
coordinateToEqualitySymbol <- optionMaybe (try (oneOf "><"))
coordinateTo <- many1 digit
string ")"
newline
return $ Coordinates (readInt coordinateFrom) coordinateFromEqualitySymbol (readInt coordinateTo) coordinateToEqualitySymbol True
setComplement :: Bool -> [Coordinates] -> [Coordinates]
setComplement complementBool coordinates = coordinatesWithComplement
where updateCoordinate complementBool coordinate = coordinate { complement = complementBool }
coordinatesWithComplement = map (updateCoordinate complementBool) coordinates
genParseGOterm :: GenParser Char st GOterm
genParseGOterm = do
many1 space
string "/GO_"
goType <- many1 (noneOf "=")
string "=\""
goId <- many1 (noneOf "-")
string "-"
goName <- many1 (noneOf "\"")
string "\""
newline
return $ GOterm goType goId goName
genParseDbXRef :: GenParser Char st DbXRef
genParseDbXRef = do
many1 space
string "/db_xref=\""
db <- many1 (noneOf ":")
string ":"
ref <- many1 (noneOf "\"")
string "\""
newline
return $ DbXRef (L.pack db) (L.pack ref)
readDouble :: String -> Double
readDouble = read
readInt :: String -> Int
readInt = read
readChar :: String -> Char
readChar = read
parseStringBracketField :: String -> GenParser Char st String
parseStringBracketField fieldname = do
many1 space
string ("/" ++ fieldname ++ "=(")
stringBracketField <- manyTill anyChar (try (string ")\n"))
return stringBracketField
parseStringField :: String -> GenParser Char st String
parseStringField fieldname = do
many1 space
string ("/" ++ fieldname ++ "=\"")
stringField <- many1( noneOf "\"")
string "\""
newline
return stringField
parseIntField :: String -> GenParser Char st Int
parseIntField fieldname = do
many1 space
string ("/" ++ fieldname ++ "=")
int <- many1 (noneOf "\n")
newline
return (readInt int)
isComplement :: Maybe String -> Bool
isComplement string
| isJust string = True
| otherwise = False